3-DIMENSIONAL STRUCTURE OF THE OLIGOSACCHARIDE TERMINUS OF GLOBOTRIAOSYLCERAMIDE AND ISOGLOBOTRIAOSYLCERAMIDE IN SOLUTION - A ROTATING-FRAME NOE STUDY USING HYDROXYL-GROUPS AS LONG-RANGE SENSORS IN CONFORMATIONAL-ANALYSIS BY H-1-NMR SPECTROSCOPY

被引：49

作者：

POPPE, L

DABROWSKI, J

VONDERLIETH, CW

KOIKE, K

OGAWA, T

机构：

[1] MAX PLANCK INST MED RES,JAHNSTR 29,W-6900 HEIDELBERG 1,GERMANY

[2] GERMAN CANC RES CTR,W-6900 HEIDELBERG 1,GERMANY

[3] INST PHYS & CHEM RES,WAKO,SAITAMA 351,JAPAN

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 189卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb15492.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spatial structures of the oligosaccharide parts of globotriaosylceramide, Gal(α1‐4)Gal(β1‐4)Glc(β1‐1)Cer (Cer = ceramide) and isoglobotriaosylceramide, Gal(α1‐3)Gal(β1‐4)Glc(β1‐1)Cer were investigated in (C2H3)2SO solution by means of laboratory and rotating frame NOE, hydroxyl protons being used as long‐range sensors defining the distance constraints. Both oligosaccharides were found to exist in more than one conformation interconverting rapidly on the NMR time scale. The conformation of the Gal(α1‐4)Gal(β1‐4)Glcβ trisaccharide dissolved in 2H2O appeared to be the same as that of the corresponding part of the glycosphingolipid in (C2H3)2SO solution. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：313 / 325

页数：13

共 60 条

[1] PREDICTION OF ANTI AND GAUCHE VICINAL PROTON-PROTON COUPLING-CONSTANTS IN CARBOHYDRATES - A SIMPLE ADDITIVITY RULE FOR PYRANOSE RINGS [J].