IDENTIFICATION OF A MINOR TG MESSENGER-RNA TRANSCRIPT IN RNA FROM NORMAL AND GOITROUS THYROIDS

被引：16

作者：

TARGOVNIK, HM

COCHAUX, P

CORACH, D

VASSART, G

机构：

[1] HOP ERASME,SERV GENET,B-1070 BRUSSELS,BELGIUM

[2] UNIV LIBRE BRUXELLES,IRIBHN,B-1070 BRUSSELS,BELGIUM

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1992年 / 84卷 / 1-2期

关键词：

THYROGLOBULIN GENE; THYROGLOBULIN MESSENGER RNA; DELETION; MOLECULAR VARIANT; THYROID; HUMAN; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0303-7207(92)90087-M

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Polymerase chain reaction (PCR) amplification of nt 4502 to nt 5184 of the thyroglobulin (Tg) mRNA from several patients, with or without elevated serum thyrotropin (TSH), showed a predominant fragment of the expected size (683 bp) and a minor fragment of 512 bp. The sequence of this minor fragment revealed that 171 bp were missing between position 4567 and 4737. It is highly probable that the deleted sequence corresponds to a complete exon, suggesting an alternative splicing as mechanism for the generation of the minor transcript.

引用

页码：R23 / R26

页数：4

共 14 条

[1]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[2]

CHRISTOPHE D, 1990, TEM SEP, P351

[3]

HENRY M, 1990, J IMMUNOL, V145, P3692

[4] THE MOLECULAR-GENETICS OF 3 THYROID AUTOANTIGENS - THYROGLOBULIN, THYROID PEROXIDASE AND THE THYROTROPIN RECEPTOR [J].