BRANCHED-CHAIN 2-OXO ACID DEHYDROGENASE INTERFERES WITH THE MEASUREMENT OF THE ACTIVITY AND ACTIVITY STATE OF HEPATIC PYRUVATE-DEHYDROGENASE

被引：12

作者：

GOODWIN, GW ^{[1
]}

PAXTON, R ^{[1
]}

GILLIM, SE ^{[1
]}

HARRIS, RA ^{[1
]}

机构：

[1] INDIANA UNIV, SCH MED, DEPT BIOCHEM, INDIANAPOLIS, IN 46223 USA

来源：

BIOCHEMICAL JOURNAL | 1986年 / 236卷 / 01期

关键词：

D O I：

10.1042/bj2360111

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxidative decarboxylation of pyruvate by branched-chain 2-oxo acid dehydrogenase can result in over-estimation of the expressed and total activity of hepatic pyruvate dehydrogenase. Pyruvate is a poor substrate for branched-chain 2-oxo acid dehydrogenase relative to the branched-chain oxo acids; however, the comparable total activities of the two complexes in liver, the much greater activity state of branched-chain 2-oxo acid dehydrogenase compared with pyruvate dehydrogenase in most physiological states, and the use of high pyruvate concentrations, explain the interference that can occur in conventional radiochemical or indicator-enzyme linked assays of pyruvate dehydrogenase. Goat antibody that specifically inhibited branched-chain 2-oxo acid dehydrogenase was used in this study to provide a more specific assay for pyruvate dehydrogenase.

引用

页码：111 / 114

页数：4

共 18 条

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