A NUCLEAR FACTOR REQUIRES ZN-2+ TO BIND A REGULATORY MRE ELEMENT OF THE MOUSE GENE ENCODING METALLOTHIONEIN-1

被引：39

作者：

SEGUIN, C ^{[1
]}

机构：

[1] UNIV LAVAL,FAC MED,DEPT PHYSIOL,QUEBEC CITY G1K 7P4,QUEBEC,CANADA

来源：

GENE | 1991年 / 97卷 / 02期

基金：

英国医学研究理事会;

关键词：

RECOMBINANT DNA; TRANS-ACTING FACTOR; CHELATOR; INDUCIBLE PROMOTER; HEAVY METAL IONS; EXONUCLEASE-III; FOOTPRINTING;

D O I：

10.1016/0378-1119(91)90066-K

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The metal ion requirement of nuclear proteins for binding to the metal regulatory element d(MREd) of the mouse gene encoding metallothionein-1 was investigated using an in vitro exonuclease III footprinting assay. The specific DNA-binding activity of the factor was inactivated by the chelating agents, EDTA and 1,10-phenanthroline. Binding activity was restored by Zn2+, but not by Cd2+. These results show that Zn2+ ions are a required component for specific in vitro DNA binding of the MREd-binding protein.

引用

页码：295 / 300

页数：6

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