TAXONOMIC SIGNIFICANCE OF DIFFERENCES IN DNA METHYLATION WITHIN THE MYCOPLASMA-MYCOIDES CLUSTER DETECTED WITH RESTRICTION ENDONUCLEASES MBOI AND DPNI

被引：8

作者：

BERGEMANN, AD ^{[1
]}

WHITLEY, JC ^{[1
]}

FINCH, LR ^{[1
]}

机构：

[1] UNIV MELBOURNE,RUSSELL GRIMWADE SCH BIOCHEM,PARKVILLE,VIC 3052,AUSTRALIA

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1990年 / 11卷 / 01期

关键词：

D O I：

10.1111/j.1472-765X.1990.tb00134.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

DNA from 22 strains belonging to the ‘Mycoplasma mycoides cluster’ was tested for methylation of adenine in GATC sequences by its resistance or susceptibility to digestion by the restriction endonucleases MboI, which is inhibited by the methylation, or DpnI, which requires the methylation. Strains of bovine serogroup 7, M. mycoides subsp. mycoides SC, and some M. mycoides subsp. capri strains plus M. capricolum strain Cal Kid lacked the methylation, whereas other strains of M. mycoides subsp. capri and of M. capricolum, plus the F38‐like and M. mycoides subsp. mycoides LC strains, possessed it. We conclude that this simple test could provide a valuable criterion in identifying these mycoplamas. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：48 / 51

页数：4

共 12 条

[1]

ANDERSEN H, 1984, J GEN MICROBIOL, V130, P1409

[2] CHARACTERIZATION OF A RESTRICTION ENDONUCLEASE FROM UREAPLASMA-UREALYTICUM 960 AND DIFFERENCES IN DEOXYRIBONUCLEIC-ACID MODIFICATION OF HUMAN UREAPLASMAS [J].

COCKS, BG ;

FINCH, LR .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1987, 37 (04) :451-453

[3] A PHYSICAL MAP OF THE GENOME OF UREAPLASMA-UREALYTICUM 960T WITH RIBOSOMAL-RNA LOCI [J].

COCKS, BG ;

PYLE, LE ;

FINCH, LR .

NUCLEIC ACIDS RESEARCH, 1989, 17 (16) :6713-6719

[4]

COSTAS M, 1987, J GEN MICROBIOL, V133, P3319

[5]

COTTEW GS, 1987, ISRAEL J MED SCI, V23, P632

[6]

LEACH RH, 1989, J GEN MICROBIOL, V135, P2993

[7]

Maniatis T., 1982, MOL CLONING

[8] A PHYSICAL MAP OF THE GENOME OF MYCOPLASMA-MYCOIDES SUBSPECIES MYCOIDES-Y WITH SOME FUNCTIONAL LOCI [J].

PYLE, LE ;

FINCH, LR .

NUCLEIC ACIDS RESEARCH, 1988, 16 (13) :6027-6039

[9] PREPARATION AND FIGE SEPARATION OF INFREQUENT RESTRICTION FRAGMENTS FROM MYCOPLASMA-MYCOIDES DNA [J].

PYLE, LE ;

FINCH, LR .

NUCLEIC ACIDS RESEARCH, 1988, 16 (05) :2263-2268

[10]

PYLE LE, 1990, UNPUB J BACTERIOLOGY, V172

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