RETINOIC ACID TREATMENT INDUCES TYPE-X COLLAGEN GENE-EXPRESSION IN CULTURED CHICK CHONDROCYTES

被引:101
作者
PACIFICI, M
GOLDEN, EB
IWAMOTO, M
ADAMS, SL
机构
[1] Department of Anatomy/Histology, School of Dental Medicine, University of Pennsylvania, Philadelphia
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0014-4827(91)90497-I
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The vitamin A derivative retinoic acid (RA) is widely thought to be involved in cartilage development, but its precise roles and mechanisms of action in this complex process remain unclear. We have tested the hypothesis that RA is involved in chondrocyte maturation during endochondral ossification and, in particular, is an inducer of maturation-associated traits such as type X collagen and alkaline phosphatase. Immature chondrocytes isolated from the caudal region of Day 19 chick embryo sterna were seeded in secondary monolayer cultures and treated either with a high dose (100 nM) or with physiological doses (10-35 nM) of RA for up to 3 days. We found that after an initial lag of about 24 h, physiological doses of RA indeed induced type X collagen gene expression in the immature cells. This induction was not accompanied by obvious changes in expression of the type II collagen and large aggregating proteoglycan core protein genes. As revealed by immunocytochemistry, 30-35% of the cells in cultures treated with RA for 3 days were engaged in type X collagen production. Interestingly, these cells were relatively similar in size to chondrocytes in which no type X collagen was detected, suggesting that chondrocytes can initiate type X collagen production independent of cell hypertrophy. RA treatment also led to increased alkaline phosphatase activity occurring as early as 24 h after the start of treatment. The data in this study indicate that RA may have a role in endochondral ossification as an inducer/promoter of maturation-associated traits during chondrocyte maturation. © 1991.
引用
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页码:38 / 46
页数:9
相关论文
共 50 条
[1]  
ALI SY, 1976, FED PROC, V35, P135
[2]  
BESSEY OA, 1946, J BIOL CHEM, V164, P321
[3]   THE CULTURE OF CHICK-EMBRYO CHONDROCYTES AND THE CONTROL OF THEIR DIFFERENTIATED FUNCTIONS INVITRO .1. CHARACTERIZATION OF THE CHONDROCYTE-SPECIFIC PHENOTYPES [J].
CAPASSO, O ;
GIONTI, E ;
PONTARELLI, G ;
AMBESIIMPIOMBATO, FS ;
NITSCH, L ;
TAJANA, G ;
CANCEDDA, R .
EXPERIMENTAL CELL RESEARCH, 1982, 142 (01) :197-206
[4]   TYPE-X COLLAGEN-SYNTHESIS BY CULTURED CHONDROCYTES DERIVED FROM THE PERMANENT CARTILAGINOUS REGION OF CHICK-EMBRYO STERNUM [J].
CASTAGNOLA, P ;
TORELLA, G ;
CANCEDDA, R .
DEVELOPMENTAL BIOLOGY, 1987, 123 (02) :332-337
[5]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[6]  
CLARK CC, 1985, COLLAGEN REL RES, V5, P205
[7]  
DOEGE K, 1986, J BIOL CHEM, V261, P8108
[8]   DIFFERENTIAL EXPRESSION OF GENES ENCODING ALPHA,BETA AND GAMMA-RETINOIC ACID RECEPTORS AND CRABP IN THE DEVELOPING LIMBS OF THE MOUSE [J].
DOLLE, P ;
RUBERTE, E ;
KASTNER, P ;
PETKOVICH, M ;
STONER, CM ;
GUDAS, LJ ;
CHAMBON, P .
NATURE, 1989, 342 (6250) :702-705
[9]   CHARACTERIZATION OF CONCENTRATION GRADIENTS OF A MORPHOGENETICALLY ACTIVE RETINOID IN THE CHICK LIMB BUD [J].
EICHELE, G ;
THALLER, C .
JOURNAL OF CELL BIOLOGY, 1987, 105 (04) :1917-1923
[10]   PHYSIOLOGICAL-ROLE OF VITAMIN-A IN GROWTH CARTILAGE CELLS - LOW CONCENTRATIONS OF RETINOIC ACID STRONGLY PROMOTE THE PROLIFERATION OF RABBIT COSTAL GROWTH CARTILAGE CELLS IN CULTURE [J].
ENOMOTO, M ;
PAN, H ;
SUZUKI, F ;
TAKIGAWA, M .
JOURNAL OF BIOCHEMISTRY, 1990, 107 (05) :743-748