A ROLE IN CELL-BINDING FOR THE C-TERMINUS OF PNEUMOLYSIN, THE THIOL-ACTIVATED TOXIN OF STREPTOCCOCCUS-PNEUMONIAE

被引：36

作者：

OWEN, RHG ^{[1
]}

BOULNOIS, GJ ^{[1
]}

ANDREW, PW ^{[1
]}

MITCHELL, TJ ^{[1
]}

机构：

[1] UNIV LEICESTER, DEPT MICROBIOL & IMMUNOL, LEICESTER LE1 9HN, ENGLAND

来源：

FEMS MICROBIOLOGY LETTERS | 1994年 / 121卷 / 02期

基金：

英国惠康基金;

关键词：

PNEUMOLYSIN; STREPTOCOCCUS PNEUMONIAE; POLYMERASE CHAIN REACTION; THIOL-ACTIVATED TOXIN; CELL BINDING;

D O I：

10.1016/0378-1097(94)90129-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Cell binding of pneumolysin to target cells is an important step in the lysis of cells by this toxin. We sought to locate the cell-binding region of pneumolysin. Deletion of the six C-terminal amino acids decreased cell-binding activity by 98%. Furthermore, mutagenesis of an amino acid near the C-terminus decreased the cell-binding activity of full-length pneumolysin by 90%. The C-terminus of pneumolysin has an important role in cell-binding activity.

引用

页码：217 / 221

页数：5

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