EXPRESSION AND SECRETION OF AN ARTHROBACTER DEXTRANASE IN THE ORAL BACTERIUM STREPTOCOCCUS-GORDONII

被引：19

作者：

KUBO, S

KUBOTA, H

OHNISHI, Y

MORITA, T

MATSUYA, T

MATSUSHIRO, A

机构：

[1] OSAKA UNIV,MICROBIAL DIS RES INST,DEPT MICROBIAL GENET,3-1 YAMADAOKA,SUITA,OSAKA 565,JAPAN

[2] OSAKA UNIV,FAC DENT,DEPT ORAL & MAXILLOFACIAL SURG 1,SUITA,OSAKA 565,JAPAN

来源：

INFECTION AND IMMUNITY | 1993年 / 61卷 / 10期

关键词：

D O I：

10.1128/IAI.61.10.4375-4381.1993

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have constructed a plasmid to express and secrete dextranase in the oral bacterium Streptococcus gordonii. The dextranase gene from Arthrobacter sp. strain CB-8 was linked to a promoter and a DNA sequence encoding the signal peptide of Streptococcus downei glucosyltransferase I (gtfI) followed by the Escherichia coli rrnBt1t2 terminator and inserted in the shuttle vector pVA838. S. gordonii transformed with this plasmid (pMNK-4) expressed and secreted mature Arthrobacter dextranase. The transformant was found to repress the firm adherence of water-insoluble glucan in a coculture experiment with cariogenic bacteria, Streptococcus sobrinus, in the presence of sucrose. Such genetically engineered oral bacteria could provide a therapy to prevent dental caries.

引用

页码：4375 / 4381

页数：7

共 31 条

[1] INVITRO STUDIES OF DENTAL PLAQUE-FORMATION - ADSORPTION OF ORAL STREPTOCOCCI TO HYDROXYAPATITE [J].