THE USE OF THE DIGITAL CELL IMAGE-ANALYSIS OF FEULGEN-STAINED NUCLEI TO DETECT APOPTOSIS

被引:14
作者
CAMBY, I
SALMON, I
DANGUY, A
PASTEELS, JL
KISS, R
机构
[1] FREE UNIV BRUSSELS,FAC MED,HISTOL LAB,B-1070 BRUSSELS,BELGIUM
[2] FREE UNIV BRUSSELS,HOP ERASME,SERV ANAT PATHOL,B-1070 BRUSSELS,BELGIUM
来源
HISTOCHEMISTRY AND CELL BIOLOGY | 1995年 / 104卷 / 05期
关键词
D O I
10.1007/BF01458135
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Cell death is an essential event in the functioning of multicellular organisms. It plays a role opposite to that of mitosis in the regulation of cell populations. In the present work, we describe an original methodology which permits the easy detection and count of apoptotic cells in a given tissue. This methodology is based on the digital cell image analysis of Feulgen stained nuclei, which also permits the calculation of the proliferation index, i.e. the percentage of cells in the S phase of the cell cycle. This percentage of cells in the S phase is strongly related to the mitotic index. Our methodology, which involves the multivariate analysis of 14 morphonuclear parameters computed by means of the digitized cell image analysis of Feulgen-stained nuclei, was applied here to a well-known biological apoptosis model, namely glucocorticoid-treated rat thymocytes. The parameters that permitted the detection of apoptotic cells were the integrated optical density, a parameter that describes the nuclear DNA content, and the run length percentage and long run length parameters which are related to the pattern of chromatin condensation. This determination can be carried out on a relatively small number of cells.
引用
收藏
页码:407 / 414
页数:8
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