ENZYME-PRODUCTION BY RECOMBINANT TRICHODERMA-REESEI STRAINS

被引：68

作者：

UUSITALO, JM ^{[1
]}

NEVALAINEN, KMH ^{[1
]}

HARKKI, AM ^{[1
]}

KNOWLES, JKC ^{[1
]}

PENTTILA, ME ^{[1
]}

机构：

[1] ALKO LTD,RES LABS,HELSINKI,FINLAND

来源：

JOURNAL OF BIOTECHNOLOGY | 1991年 / 17卷 / 01期

关键词：

ABBREVIATIONS; ELISA; ENZYME LINKED IMMUNOSORBENT ASSAY; PAGE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PMSF; PHENYLMETHYLSULFONYL FLUORIDE; SDS; SODIUM DODECYL SULFATE; TCA; TRICHLOROACETIC ACID;

D O I：

10.1016/0168-1656(91)90025-Q

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The production of both homologous and heterologous proteins with the cellulolytic filamentous fungus Trichoderma reesei is described. Biotechnically important improvements in the production of cellulolytic enzymes have been obtained by genetic engineering methodology to construct strains secreting novel mixtures of cellulases. These improvements have been achieved by gene inactivation and promoter changes. The strong and highly inducible promoter of the gene encoding the major cellulase, cellobiohydrolase I (CBHI) has also been used for the production of eukaryotic heterologous proteins in Trichoderma. The expression and secretion of active calf chymosin is described in detail.

引用

页码：35 / 49

页数：15

共 52 条

[1] INDUCTION, ISOLATION AND TESTING OF STABLE TRICHODERMA-REESEI MUTANTS WITH IMPROVED PRODUCTION OF SOLUBILIZING CELLULASE [J].