RECOVERY OF AN EXTRACELLULAR ALKALINE PROTEASE FROM WHOLE FERMENTATION BROTH USING REVERSED MICELLES

被引：106

作者：

RAHAMAN, RS ^{[1
]}

CHEE, JY ^{[1
]}

CABRAL, JMS ^{[1
]}

HATTON, TA ^{[1
]}

机构：

[1] MIT,DEPT CHEM ENGN,CAMBRIDGE,MA 02139

来源：

BIOTECHNOLOGY PROGRESS | 1988年 / 4卷 / 04期

关键词：

Chemical Operations--Extraction - Chemical Reactions--Fermentation - Solvents - Surface Active Agents--Solutions;

D O I：

10.1002/btpr.5420040405

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The liquid-liquid extraction of an extracellular alkaline protease from its fermentation broth was carried out, using a reversed micellar oganic solvent. Optimum process parameters were determined for extraction using a 250 mM AOT in isooctane micellar solution. With unequal phase volume ratios, purification factors as high as 6 have been obtained from completely untreated broth. Yields up to 56% were achieved from the same broth using a three-stage cascade.

引用

页码：218 / 224

页数：7