FLUIDIZED-BED REACTORS OPERATING WITH IMMOBILIZED ENZYME-SYSTEMS - DESIGN-MODEL AND ITS EXPERIMENTAL-VERIFICATION

被引：22

作者：

BODALO, A

GOMEZ, JL

GOMEZ, E

BASTIDA, J

MAXIMO, MF

机构：

[1] Departamento de Ingeniería Química, Grupo de Ingeniería Bioquímica, Facultad de Química, Murcia, Campus de Espinardo

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1995年 / 17卷 / 10期

关键词：

FLUIDIZED BED REACTOR; IMMOBILIZED ENZYMES; REVERSIBLE MICHAELIS-MENTEN; PRODUCT INHIBITION; DIFFUSIONAL LIMITATIONS;

D O I：

10.1016/0141-0229(94)00125-B

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A general model for the design of fluidized bed reactors operating with immobilized enzymes in spherical porous particles is presented. It was solved for monosubstrate reactions following reversible Michaelis-Menten kinetics, as well as competitive inhibition by the product. The general model also allows the evaluation of irreversible and/or non-inhibited kinetics. To check the model, experimental conversions obtained in a fluidized bed reactor were compared to those predicted theoretically. The chemical process used for the validation of the model was the hydrolysis of o-nitrophenyl-beta,o-galactopyranoside catalyzed by beta-galactosidase covalently immobilized in Chromosorb-W. The model was tested in 160 different experimental conditions.

引用

页码：915 / 922

页数：8

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