ALPHA2-MACROGLOBULIN BINDING OF TRYPSIN CHYMOTRYPSIN PAPAIN AND CATIONIC ASPARTATE AMINOTRANSFERASE

Ultracentrifugal experiments indicate that the α2-macroglobulin binding of cationic aspartate aminotransferase, trypsin, and chymotrypsin occurs in conditions approaching physiological. The order of affinity for α2-macroglobulin is trypsin > chymotrypsin > cationic aspartate aminotransferase. The binding of cationic aspartate aminotransferase is sensitive to changes in ionic concentration. In general, binding is diminished as the salt concentration rises, but borate in dilute solution enhances the binding effect, and so does a serum factor which is dialysable, ultrafiltrable, and extractable by ether from acidified serum ultrafiltrate. © 1968.