THE POSITIVE-INSIDE RULE APPLIES TO THYLAKOID MEMBRANE-PROTEINS

被引：78

作者：

GAVEL, Y

STEPPUHN, J

HERRMANN, R

VONHEIJNE, G

机构：

[1] KAROLINSKA INST,CTR BIOTECHNOL,NOVUM,DEPT MOLEC BIOL,S-14157 HUDDINGE,SWEDEN

[2] ROYAL INST TECHNOL,DEPT THEORET PHYS,THEORET BIOPHYS RES GRP,S-10044 STOCKHOLM 70,SWEDEN

[3] UNIV MUNICH,INST BOT,W-8000 MUNICH 19,GERMANY

来源：

FEBS LETTERS | 1991年 / 282卷 / 01期

关键词：

THYLAKOID; MEMBRANE PROTEIN; TOPOLOGY; POSITIVE INSIDE RULE;

D O I：

10.1016/0014-5793(91)80440-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In integral membrane proteins, regions that span the lipid bilayer alternate with regions that are exposed on either side of the membrane. For proteins form the plasma membrane of both prokaryotic and eukaryotic cells it has been shown that the exposed parts follow a 'positive-inside rule': on average, segments that are translocated across the membrane have a 2-4-fold lower frequency of positively charged residues than nontranslocated segments. We now present an analysis of proteins from the thylakoid membrane of chloroplasts. It is shown that these proteins have the same charge asymmetry as has been reported for proteins from other membrane systems, with their more highly charged regions facing the stromal compartment.

引用

页码：41 / 46

页数：6

共 48 条

[1] NUCLEOTIDE-SEQUENCE OF THE CLUSTERED GENES FOR THE 44-KD CHLOROPHYLL-A APOPROTEIN AND THE 32KD-LIKE PROTEIN OF THE PHOTOSYSTEM-II REACTION CENTER IN THE SPINACH PLASTID CHROMOSOME [J].