GENE FOR THE MAJOR ANTIGENIC STRUCTURAL PROTEIN-(P100) OF HUMAN HERPESVIRUS-6

被引:39
作者
NEIPEL, F
ELLINGER, K
FLECKENSTEIN, B
机构
关键词
D O I
10.1128/JVI.66.6.3918-3924.1992
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A human herpesvirus 6 (HHV-6) structural protein of 100 kDa (p100) is the polypeptide most frequently and intensively reactive in immunoblotting analyses with human sera on HHV-6-infected cells or partially purified virions. The gene for p100 was identified by screening a bacteriophage lambda library with monospecific rabbit antisera. The gene codes for a polypeptide of 870 amino acids with a calculated molecular size of 97 kDa. Its amino-terminal third is weakly homologous to the immunogenic basic matrix phosphoprotein pp150 of human cytomegalovirus. Five fragments representing more than 93% of HHV-6 p100 were prokaryotically expressed. The antigenic epitopes of p100 were preliminarily mapped by immunoblotting with human sera. They are located within the carboxy-terminal part which is neither homologous nor cross-reactive to pp150 of human cytomegalovirus. Availability of the gene for the immunodominant structural protein should provide tools for studies of pathogenesis by HHV-6.
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页码:3918 / 3924
页数:7
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