SPECIFIC REGION OF THE C-MYC PROMOTER IS RESPONSIVE TO ELECTRIC AND MAGNETIC-FIELDS

被引：42

作者：

LIN, H

GOODMAN, R

SHIRLEYHENDERSON, A

机构：

[1] COLUMBIA UNIV,DEPT PATHOL,NEW YORK,NY 10032

[2] CUNY HUNTER COLL,INST BIOMOLEC STRUCT & FUNCT,GRAD DIV,NEW YORK,NY 10021

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 1994年 / 54卷 / 03期

关键词：

C-MYC PROMOTER; CAT EXPRESSION; LOW FREQUENCY ELECTRIC AND EM FIELDS; TRANSCRIPTION;

D O I：

10.1002/jcb.240540304

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The level of c-myc transcripts is increased in cells exposed to extremely low frequency (elf) electromagnetic (EM) fields at 60 Hz. The aim of the present experiments was to determine if regulatory regions upstream of the c-myc gene modulate the response to EM fields. DNA upstream of P1 of both mouse and human c-myc genes was transfected into cells as CAT constructs. The presence of DNA 5' to the human or mouse myc genes results in increased expression of CAT following 20 min exposures of cells to 60 Hz elf EM fields. Specific portions of the human upstream DNA were deleted and introduced into cells. The region responsive to EM fields is located between -353 and -1,257 relative to the P-1 promoter. (C) 1994 Wiley-Liss, Inc.

引用

页码：281 / 288

页数：8

共 31 条

[1] EFFECTS OF WEAK AMPLITUDE-MODULATED MICROWAVE FIELDS ON CALCIUM EFFLUX FROM AWAKE CAT CEREBRAL-CORTEX [J].