MICELLE STABILIZED PHOSPHORESCENT IMMUNOASSAY BASED ON BISPECIFIC ANTIBODIES AGAINST LABEL AND ANTIGEN

被引：11

作者：

DEMCHEVA, MV

MANTROVA, EY

SAVITSKY, A

BEHRSING, O

MICHEEL, B

HEMMILA, I

机构：

[1] AN BAKH BIOCHEM INST,MOSCOW 117071,RUSSIA

[2] INST MOLEC BIOL,O-1115 BERLIN,GERMANY

[3] WALLAC OY,SF-20101 TURKU,FINLAND

来源：

ANALYTICAL LETTERS | 1995年 / 28卷 / 02期

关键词：

PHOSPHORESCENCE; PD-COPROPORPHYRIN; HCG; HYBRID HYBRIDOMA; BIFUNCTIONAL MONOCLONAL ANTIBODY;

D O I：

10.1080/00032719508000319

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Hybrid hybridomas producing bispecific antibodies against alpha-subunit of hCG and Pd-coproporphyrin are obtained by using FAGS. Conjugates of Pd-coproporphyrin with different proteins are tested for better sensitivity in micelle stabilized phosphorescent immunoassay. The role of steric factors for interaction of immobilized antibodies with antigens are discussed.

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页码：249 / 258

页数：10

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