STUDIES OF THE HETEROGENEITY OF A CANDIDA-CYLINDRACEA (RUGOSA) LIPASE - MONITORING OF ESTEROLYTIC ACTIVITY AND ENANTIOSELECTIVITY BY CHIRAL LIQUID-CHROMATOGRAPHY

被引：19

作者：

ALLENMARK, S ^{[1
]}

OHLSSON, A ^{[1
]}

机构：

[1] GOTHENBURG UNIV, MICROBIOL CHEM LAB, S-41346 GOTHENBURG, SWEDEN

来源：

BIOCATALYSIS | 1992年 / 6卷 / 03期

关键词：

LIPASE; ESTER HYDROLYSIS; ENANTIOSELECTIVITY; CHIRAL LIQUID CHROMATOGRAPHY; CANDIDA-CYLINDRACEA (RUGOSA);

D O I：

10.3109/10242429209014897

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the determination of lipase activity in terms of rate and enantioselectivity of hydrolysis of a chiral ester substrate has been developed. When this method was applied to fractions, isolated from preparative, column chromatographic separations (anion-exchange, molecular sieve) of the lipase, significant differences in enantioselectivity (E) was found between the fractions. The highest enantioselectivity was found in the first main peak obtained on DEAE-Sepharose chromatography, meaning that the enzyme with the highest isoelectric point shows the highest esterolytic enantioselectivity. The experimental results are discussed in the light of some earlier reported results and with respect to the possible existence of subunit aggregates and isoenzymes. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：211 / 221

页数：11

共 19 条

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