CARBOHYDRATES IN MAMMALIAN TRYPTOPHANYL-TRANSFER RNA-SYNTHETASE

被引：11

作者：

KOVALEVA, GK ^{[1
]}

ZHELTOVA, AO ^{[1
]}

NIKITUSHKINA, TV ^{[1
]}

EGOROV, TA ^{[1
]}

MUSOLJAMOV, AC ^{[1
]}

KISSELEV, LL ^{[1
]}

机构：

[1] ND ZELINSKII ORGAN CHEM INST, MOSCOW 117334, USSR

来源：

FEBS LETTERS | 1992年 / 309卷 / 03期

关键词：

TRYPTOPHANYL-TRANSFER RNA SYNTHETASE; BEEF; SUGAR; GLYCOPROTEIN; MAMMAL; AMINOACYL-TRANSFER RNA SYNTHETASE;

D O I：

10.1016/0014-5793(92)80802-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Homogeneous preparations of bovine tryptophanyl-tRNA synthetase (EC 6.1.1.2) contain monosaccharides (mannose, fucose, galactose, N-acetylglucosamine) as revealed by liquid chromatography. Their content comprises 2.5-3.0% (w/w) of the enzyme composed of two subunits (60 kDa x 2). The same set of sugars was detected in elastase and CNBr-generated fragments (with molecular masses of approx. 40 kDa and 30 kDa, respectively). It is concluded that bovine tryptophanyl-tRNA synthetase, in addition to being a metallo- and phosphoprotein, is also a glycoprotein.

引用

页码：337 / 339

页数：3

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