ISOLATION OF ARABIDOPSIS-THALIANA MUTANTS HYPERSENSITIVE TO GAMMA-RADIATION

被引：27

作者：

DAVIES, C ^{[1
]}

HOWARD, D ^{[1
]}

TAM, G ^{[1
]}

WONG, N ^{[1
]}

机构：

[1] MCGILL UNIV,DEPT BIOL,MONTREAL H3A 1B1,PQ,CANADA

来源：

MOLECULAR & GENERAL GENETICS | 1994年 / 243卷 / 06期

关键词：

ARABINOPSIS THALIANA; RAD MUTANTS; GAMMA RADIATION; UV RADIATION; DNA REPAIR;

D O I：

10.1007/BF00279575

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A screening method for mutants of Al Arabidopsis thaliana hypersensitive to gamma-radiation has been devised. Plants grown from ethyl methanesulfonate (EMS)-treated seeds were irradiated at the seedling stage, which is highly radiosensitive due to extensive cell division. Severe growth inhibition of mutant plants by a gamma-ray dose which only slightly affects wild-type plants was the selective criterion. Twelve true-breeding hypersensitive lines were isolated from a total of 3394 screened plants. Genetic analysis of five of the lines revealed five new genes, designated RAD1-RAD5. These Arabidopsis RAD mutants are phenotypically similar to mutants in the RAD52 epistasis group of Saccharomyces cerevisiae, which are highly sensitive to ionizing radiation but not hypersensitive to UV light. One possibility is that the Arabidopsis mutants are defective in a nonhomologous or illegitimate recombination mechanism used by plants for repair of chromosome breaks.

引用

页码：660 / 665

页数：6

共 21 条

[1] ANALYSIS OF WILD-TYPE AND RAD50 MUTANTS OF YEAST SUGGESTS AN INTIMATE-RELATIONSHIP BETWEEN MEIOTIC CHROMOSOME SYNAPSIS AND RECOMBINATION [J].