SINGLE-CHANNEL ACTIVITY CORRELATED WITH MEDIUM-DURATION, CA-DEPENDENT K-CURRENT IN CULTURED RAT HIPPOCAMPAL-NEURONS

被引：22

作者：

ALGER, BE ^{[1
]}

SIM, JA ^{[1
]}

BROWN, DA ^{[1
]}

机构：

[1] UCL, DEPT PHARMACOL, LONDON, ENGLAND

来源：

NEUROSCIENCE LETTERS | 1994年 / 168卷 / 1-2期

关键词：

AFTERHYPERPOLARIZATION; TISSUE CULTURE; CALCIUM; POTASSIUM; PATCH CLAMP; VOLTAGE CLAMP;

D O I：

10.1016/0304-3940(94)90407-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Whole-cell voltage- and patch-clamp techniques were used to record the calcium-dependent component of I-mAHP and correlated single-channel activity in postnatal cultured rat hippocampal neurons. The Ca-dependent I-mAHP was elicited by voltage steps to + 10 mV from a holding potential of -50 mV in the whole-cell mode. In cell-attached patches, single currents of similar to 2 pA were observed following spontaneous action currents. The duration of ensemble-averaged single-channel activity was very similar to that of the whole-cell I-mAHP similar to 100 ms. Both whole-cell current and single-channel activity could be blocked by bath-applied cadmium, 200 mu M. Vigorous channel activity was evoked by bursts of action potentials that are known to elicit Ca-dependent AHPs. We conclude that this channel is a candidate for mediating I-mAHP.

引用

页码：23 / 28

页数：6

共 22 条

[1] EPILEPTIFORM BURST AFTERHYPERPOLARIZATION - CALCIUM-DEPENDENT POTASSIUM POTENTIAL IN HIPPOCAMPAL CA1-PYRAMIDAL CELLS [J].