MICROBIAL TRANSFORMATION OF BETA-SITOSTEROL AND STIGMASTEROL INTO 26-OXYGENATED DERIVATIVES

被引：15

作者：

AMBRUS, G ^{[1
]}

ILKOY, E ^{[1
]}

JEKKEL, A ^{[1
]}

HORVATH, G ^{[1
]}

BOCSKEI, Z ^{[1
]}

机构：

[1] CHINOIN PHARMACEUT,BUDAPEST,HUNGARY

来源：

STEROIDS | 1995年 / 60卷 / 09期

关键词：

BETA-SITOSTEROL; MICROBIAL SIDE CHAIN DEGRADATION; MECHANISM OF C-26-OXIDATION;

D O I：

10.1016/0039-128X(95)00077-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genetically modified Mycobacterium sp. BCS 396 strain has been used to transform sterols with stigmastane side chain in order to obtain 26-oxidized metabolites. beta-Sitosterol (I) was transformed to 4-stigmasten-3-one (II), 26-hydroxy-4-stigmasten-3-one (III), and 3-oxo-4-stigmasten-26-oic acid (IV), while stigmasterol (V) was converted to 4,22-stigmastadien-3-one (VI), 6 beta-hydroxy-4,22-stigmastadien-3-one (VII), 26-hydroxy-4,22-stigmastadien-3-one (VIII), 3-oxo-4,22-stigmastadien-26-oic acid methyl ester (IX), and 3-oxo-1,4,22-stigmastatrien-26-oic acid methyl ester (X) with that strain. In both beta-sitosterol and stigmasterol, 26-oxidation generates the R-configuration on C-25.

引用

页码：621 / 625

页数：5

共 17 条

[1] HYDROGENATION OF UNSATURATED STEROID RING SYSTEMS WITH MYCOBACTERIUM PHLEI [J].