EVOLUTIONARY CONSERVATION OF THE SPLICEOSOMAL PROTEIN, U2B

被引：36

作者：

SIMPSON, GG ^{[1
]}

VAUX, P ^{[1
]}

CLARK, G ^{[1
]}

WAUGH, R ^{[1
]}

BEGGS, JD ^{[1
]}

BROWN, JWS ^{[1
]}

机构：

[1] UNIV EDINBURGH,INST CELL & MOLEC BIOL,EDINBURGH EH9 3JR,MIDLOTHIAN,SCOTLAND

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 19期

关键词：

D O I：

10.1093/nar/19.19.5213

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

U1 and U2snRNPs play key roles in pre-mRNA splicing. The interactions between the U1 and U2snRNP-specific proteins, U1A, U2A' and U2B" and their respective UsnRNAs are of interest both to elucidate their roles in splicing, and as models to study RNA-protein interactions. We have cloned a full-length cDNA, encoding U2B", from potato. This is the first report of a sequence for a plant UsnRNP protein. The plant U2B" sequence exhibits extensive similarity with the human U2B" protein at both the DNA and amino acid levels. The evolutionary conservation at the protein level, particularly in sequences implicated in determining specific binding to U2snRNA, suggests conservation of U2B" function from plants to man. The significance of amino acid substitutions in the RNP-80 motif with respect to U2snRNA binding in plants is discussed.

引用

页码：5213 / 5217

页数：5

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