CIRCULATING NEUROPEPTIDE-Y IN DOG PLASMA CONSISTS OF MULTIPLE PEPTIDE-FRAGMENTS

被引：3

作者：

DESSENANAYAKE, P

WARNER, MR

BROSNIHAN, KB

LEVY, MN

FERRARIO, CM

机构：

[1] INDIANA UNIV, SCH MED, KRANNERT INST CARDIOL, DEPT PHARMACOL, INDIANAPOLIS, IN 46202 USA

[2] INDIANA UNIV, SCH MED, KRANNERT INST CARDIOL, DEPT MED, INDIANAPOLIS, IN 46202 USA

[3] MT SINAI MED CTR, DIV INVEST MED, CLEVELAND, OH 44106 USA

[4] BOWMAN GRAY SCH MED, DIV SURG SCI, WINSTON SALEM, NC 27157 USA

来源：

PEPTIDES | 1992年 / 13卷 / 06期

关键词：

HUMAN NEUROPEPTIDE-Y(1-36); PORCINE NEUROPEPTIDE-Y(1-36); OXIDIZED HUMAN NEUROPEPTIDE-Y(1-36); NEUROPEPTIDE-Y FRAGMENTS; HPLC; RADIOIMMUNOASSAY;

D O I：

10.1016/0196-9781(92)90024-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neuropeptide Y-like immunoreactivity (NPY-LI) in dog plasma was characterized and quantified using three extraction methods (Sep-Pak:acetonitrile, HCl:ethanol, and ethanol). Sep-Pak extraction yields the best recovery and preserves the integrity of the peptide. Oxidized NPY is not generated during blood collection. Using two antisera of different specificities, at least three peptide forms in normal dog arterial and venous plasma were detected. A peptide with retention times similar to oxidized NPY or peptide YY is the major component of plasma NPY-LI under basal conditions, but NPY(I-36) predominates during sympathetic stimulation. The mature peptide in dog plasma is similar to human NPY. The antiserum ABII provides a more accurate measure of circulating NPY(1-36) and its oxidized fomi. The antiserum ABI is useful for detecting NPY-like fragments.

引用

页码：1165 / 1173

页数：9

共 25 条

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