EXPRESSION OF A POKEWEED ANTIVIRAL PROTEIN IN ESCHERICHIA-COLI AND ITS CHARACTERIZATION

被引：14

作者：

KATAOKA, J

AGO, H

HABUKA, N

FURUNO, M

MASUTA, C

MIYANO, M

KOIWAI, A

机构：

[1] Life Science Research Laboratory, Japan Tobacco Inc., Midori-ku, Yokohama, Kanagawa, 227, 6-2, Umegaoka

来源：

FEBS LETTERS | 1993年 / 320卷 / 01期

关键词：

ALPHA-POKEWEED ANTIVIRAL PROTEIN; EXPRESSION; RNA N-GLYCOSIDASE ACTIVITY;

D O I：

10.1016/0014-5793(93)81651-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two expression vectors were constructed to produce a putative mature alpha-pokeweed antiviral protein (alpha-PAP) in Escherichia coli with its NH2- and COOH-terminal extrapeptides excised. One was for its intracellular expression with a methionine at its NH2-terminal. The other was for its secretion using an ompA signal peptide. The former product was purified from the total soluble proteins of the transformant with a yield of 1.74 mg/liter and the latter had a yield of 5.55 mg/liter. Both products exhibited RNA N-glycosidase activity on wheat ribosomes and inhibitory activity to protein synthesis in a rabbit reticulocyte system.

引用

页码：31 / 34

页数：4

共 22 条

[1] INHIBITION OF HERPES-SIMPLEX VIRUS MULTIPLICATION BY THE POKEWEED ANTI-VIRAL PROTEIN [J].