INSECT IMMUNITY - A TRANSGENIC ANALYSIS IN DROSOPHILA DEFINES SEVERAL FUNCTIONAL DOMAINS IN THE DIPTERICIN PROMOTER

被引:71
作者
MEISTER, M
BRAUN, A
KAPPLER, C
REICHHART, JM
HOFFMANN, JA
机构
[1] Inst. de Biol. Molec. et Cellulaire, UPR 9022 du CNRS, 67084 Strasbourg Cedex
关键词
DIPTERICIN PROMOTER; INSECT IMMUNITY; KAPPA-B-RELATED; TRANSGENIC DROSOPHILA;
D O I
10.1002/j.1460-2075.1994.tb06941.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Diptericins are antibacterial polypeptides which are strongly induced in the fat body and blood cells of dipteran insects in response to septic injury. The promoter of the single-copy, intronless diptericin gene of Drosophila contains several nucleotide sequences homologous to mammalian cis-regulatory motifs involved in the control of acute phase response genes. Extending our previous studies on the expression of the diptericin gene, we now report a quantitative analysis of the contribution of various putative regulatory elements to the bacterial inducibility of this gene, based on the generation of 60 transgenic fly lines carrying different elements fused to a reporter gene, Our data definitively identify two kappa B-related motifs in the proximal promoter as the sites conferring inducibility and tissue-specific expression to the diptericin gene. These motifs alone, however, mediate only minimal levels of expression. Additional proximal regulatory elements are necessary to attain some 20% of the full response and we suspect a role for sequences homologous to mammalian IL6 response elements and interferon-gamma responsive sites in this up-regulation. The transgenic experiments also reveal the existence of a distal regulatory element located upstream of -0.6 kb which increases the level of expression by a factor of five.
引用
收藏
页码:5958 / 5966
页数:9
相关论文
共 29 条
  • [1] ANTIBACTERIAL PEPTIDES - KEY COMPONENTS NEEDED IN IMMUNITY
    BOMAN, HG
    [J]. CELL, 1991, 65 (02) : 205 - 207
  • [2] REMOTE REGULATORY SEQUENCES OF THE DROSOPHILA GLUE GENE SGS3 AS REVEALED BY P-ELEMENT TRANSFORMATION
    BOUROUIS, M
    RICHARDS, G
    [J]. CELL, 1985, 40 (02) : 349 - 357
  • [3] THE INDUCIBLE ANTIBACTERIAL PEPTIDES OF INSECTS
    COCIANCICH, S
    BULET, P
    HETRU, C
    HOFFMANN, JA
    [J]. PARASITOLOGY TODAY, 1994, 10 (04): : 132 - 139
  • [4] KAPPA-B-LIKE MOTIFS REGULATE THE INDUCTION OF IMMUNE GENES IN DROSOPHILA
    ENGSTROM, Y
    KADALAYIL, L
    SUN, SC
    SAMAKOVLIS, C
    HULTMARK, D
    FAYE, I
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1993, 232 (02) : 327 - 333
  • [5] GATEFF E, 1978, SCIENCE, V200, P1448, DOI 10.1126/science.96525
  • [6] INSECT IMMUNITY - THE DIPTERICIN PROMOTER CONTAINS MULTIPLE FUNCTIONAL REGULATORY SEQUENCES HOMOLOGOUS TO MAMMALIAN ACUTE-PHASE RESPONSE ELEMENTS
    GEORGEL, P
    MEISTER, M
    KAPPLER, C
    LEMAITRE, B
    REICHHART, JM
    HOFFMANN, JA
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 197 (02) : 508 - 517
  • [7] SGS-3 TRANSCRIPT LEVELS ARE DETERMINED BY MULTIPLE REMOTE SEQUENCE ELEMENTS
    GIANGRANDE, A
    METTLING, C
    RICHARDS, G
    [J]. EMBO JOURNAL, 1987, 6 (10) : 3079 - 3084
  • [8] CELL-TYPE SPECIFIC ACTIVITY OF 2 GLUCOCORTICOID RESPONSIVE UNITS OF RAT TYROSINE AMINOTRANSFERASE GENE IS ASSOCIATED WITH MULTIPLE BINDING-SITES FOR C/EBP AND A NOVEL LIVER-SPECIFIC NUCLEAR FACTOR
    GRANGE, T
    ROUX, J
    RIGAUD, G
    PICTET, R
    [J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (01) : 131 - 139
  • [9] CONTROL ELEMENTS OF THE DROSOPHILA SEGMENTATION GENE FUSHI-TARAZU
    HIROMI, Y
    KUROIWA, A
    GEHRING, WJ
    [J]. CELL, 1985, 43 (03) : 603 - 613
  • [10] SYNERGISTIC ACTION OF INTERLEUKIN-6 AND GLUCOCORTICOIDS IS MEDIATED BY THE INTERLEUKIN-6 RESPONSE ELEMENT OF THE RAT ALPHA(2) MACROGLOBULIN GENE
    HOCKE, GM
    BARRY, D
    FEY, GH
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (05) : 2282 - 2294