MEMBRANE LATERAL PRESSURE AS A MODULATOR OF GLYCEROL-3-PHOSPHATE DEHYDROGENASE-ACTIVITY

被引：16

作者：

AMLER, E

JASINSKA, R

DRAHOTA, Z

ZBOROWSKI, J

机构：

[1] M NENCKI INST EXPTL BIOL, DEPT CELLULAR BIOCHEM, PASTEURA 3, PL-02093 WARSAW, POLAND

[2] CZECHOSLOVAK ACAD SCI, INST PHYSIOL, CS-14220 PRAGUE 4, CZECHOSLOVAKIA

来源：

FEBS LETTERS | 1990年 / 271卷 / 1-2期

关键词：

Brown adipose tissue mitochondrion; Förster energy transfer; Glycerol-3-phosphate dehydrogenase; Membrane lateral pressure; Model membrane; Surface charge;

D O I：

10.1016/0014-5793(90)80398-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Michaelis-Menten kinetics of glycerol-3-phosphate dehydrogenase activity in proteoliposomes from brown adipose tissue mitochondria with exogenously added phospholipids or cholesterol was measured. It was shown that changes in membrane lipid composition affected the membrane lateral pressure and therefore modulated the enzyme activity, namely Vmax value. Contrarily, changes in surface charge caused by minute amounts of phosphatidylserine or charged organic substances influenced only the apparent Km value. The role of bulk phospholipids in regulation of glycerol-3-phosphate dehydrogenase is discussed. © 1990.

引用

页码：165 / 168

页数：4

共 28 条

[1] MG-2+-INDUCED CHANGES OF LIPID ORDER AND CONFORMATON OF (NA++K+)-ATPASE [J].