AMINO-ACID-ANALYSIS USING AMINE-SENSITIVE MEMBRANE ELECTRODES

被引：20

作者：

KATSU, T

KAYAMOTO, T

FUJITA, Y

机构：

[1] Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama

来源：

ANALYTICA CHIMICA ACTA | 1990年 / 239卷 / 01期

关键词：

Amino acids; Enzyme reactions; Membrane electrodes; Phenylalanine; Tyrosine;

D O I：

10.1016/S0003-2670(00)83831-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for determining specific amino acids is described, based on the detection of the amine formed by the enzymatic reaction of an amino acid with decarboxylase, using an amine-sensitive membrane electrode. l-Tyrosine and l-phenylalanine are considered as examples. The corresponding amines tyramine and phenethylamine, respectively, were selectively detected by using a poly(vinyl chloride)-based membrane electrode containing sodium tetrakis[3,5- bis(trifluoromethyl)phenyl]borate as an ion exchanger and tricresyl phosphate as a solvent mediator. The detection limits of l-tyrosine and l-phenylalanine were 20 and 50μM, respectively. The response characteristics of electrodes were compared by changing ion exchangers and solvent mediators. © 1990.

引用

页码：23 / 27

页数：5

共 10 条

[1]

BAUM G, 1970, ANAL LETT, V3, P105

[2] POLYMER MEMBRANE ELECTRODES .1. CHOLINE ESTER-SELECTIVE ELECTRODE [J].