DEVELOPMENTAL NEUROTROPHIN EXPRESSION IN SLICE CULTURES OF RAT HIPPOCAMPUS

被引：27

作者：

FORSTER, E ^{[1
]}

OTTEN, U ^{[1
]}

FROTSCHER, M ^{[1
]}

机构：

[1] UNIV BASEL, DEPT PHYSIOL, CH-4051 BASEL, SWITZERLAND

来源：

NEUROSCIENCE LETTERS | 1993年 / 155卷 / 02期

关键词：

BRAIN-DERIVED NEUROTROPHIC FACTOR; NERVE GROWTH FACTOR; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; IN-VITRO MODEL;

D O I：

10.1016/0304-3940(93)90711-S

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Using reverse transcription in combination with the polymerase chain reaction, the developmental expression of neurotrophins in organotypic slice cultures of rat hippocampus was investigated. Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) mRNA levels after different time periods in vitro were compared with equivalent developmental stages in vivo. Our results show that neurotrophin expression occurs in hippocampal slice cultures with a similar time course as observed in the developing hippocampus in vivo. Thus, the development of neurotrophin expression in the hippocampus does not seem to be dependent on specific extrinsic afferents.

引用

页码：216 / 219

页数：4

共 24 条

[1] A REPRODUCIBLE MICROANALYTICAL METHOD FOR THE DETECTION OF SPECIFIC RNA SEQUENCES BY DOT BLOT HYBRIDIZATION [J].