LOCALIZATION OF NEWLY SYNTHESIZED VIMENTIN SUBUNITS REVEALS A NOVEL MECHANISM OF INTERMEDIATE FILAMENT ASSEMBLY

被引：102

作者：

NGAI, J ^{[1
]}

COLEMAN, TR ^{[1
]}

LAZARIDES, E ^{[1
]}

机构：

[1] CALTECH, DIV BIOL, PASADENA, CA 91125 USA

来源：

CELL | 1990年 / 60卷 / 03期

关键词：

D O I：

10.1016/0092-8674(90)90593-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have assessed the mechanism of intermediate filament assembly by assaying the sites of incorporation of chicken vimentin subunits expressed under the control of an inducible promoter in transfected mouse fibroblasts. The localization of newly synthesized vimentin was determined by immunofluorescence and immunoelectron microscopy at short time periods of induced synthesis, using antibodies specific for chicken vimentin. Under conditions where neither the soluble subunit pools nor the steady-state distribution of endogenous filaments are affected, newly synthesized vimentin incorporates into the vimentin filament network at numerous and discrete sites throughout the cell. Over time, the pattern of newly assembled vimentin converts to a continuous array coincident with preexisting vimentin filaments. These results are consistent with a novel mechanism of intermediate filament assembly, whereby growth of intermediate filaments occurs by topographically restricted and localized subunit addition, necessitating a transient disruption of filament integrity. © 1990.

引用

页码：415 / 427

页数：13

共 82 条

[71] ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS [J].