CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES OF RECOMBINANT HUMAN GRANULOCYTE-STIMULATING FACTOR (KW2228)

被引：7

作者：

NAGAHARA, Y ^{[1
]}

KONISHI, N ^{[1
]}

YOKOO, Y ^{[1
]}

HIRAYAMA, N ^{[1
]}

机构：

[1] KYOWA HAKKO KOGYO CO LTD,TOKYO RES LABS,3-6-6 ASAHIMACHI,TOKYO 194,JAPAN

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 214卷 / 01期

关键词：

D O I：

10.1016/0022-2836(90)90143-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human granulocyte colony-stimulating factor (hG-CSF) specifically stimulates proliferation of neutrophils. Two crystal forms of a mutant of hG-CSF expressed inEscherichia coli have been obtained using the hanging drop vapour diffusion method. One form is triclinic, space groupP1, with cell dimensionsa = 37.3A °, b = 46.4A °, c = 47.7A °, α = 105.5 °, β = 98.0 ° and γ = 109.4 °. The other is monoclinic, space groupC2, with cell dimensionsa = 82.0A °, b = 49.2A °, c = 49.4Å and β = 113.9 °. Both crystal forms diffract beyond 2.0A °and are suitable for X-ray analysis. © 1990 Academic Press Limited.

引用

页码：25 / 26

页数：2

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