AUTOANTIBODIES TO TOPOISOMERASE-I (SCL-70) - ANALYSIS BY GEL-DIFFUSION, IMMUNOBLOT, AND ENZYME-LINKED-IMMUNOSORBENT-ASSAY

被引：44

作者：

HILDEBRANDT, S

WEINER, ES

SENECAL, JL

NOELL, GS

EARNSHAW, WC

ROTHFIELD, NF

机构：

[1] HOP NOTRE DAME DE BON SECOURS,RHEUMATOL IMMUNOL RES LAB,MONTREAL H2L 4K8,QUEBEC,CANADA

[2] HOP NOTRE DAME DE BON SECOURS,DIV RHEUMATOL,CONNECT TISSUE DIS CLIN,MONTREAL H2L 4K8,QUEBEC,CANADA

[3] UNIV MONTREAL,SCH MED,MONTREAL H3C 3J7,QUEBEC,CANADA

[4] JOHNS HOPKINS UNIV,DEPT CELL BIOL & ANAT,BALTIMORE,MD 21218

来源：

CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY | 1990年 / 57卷 / 03期

关键词：

D O I：

10.1016/0090-1229(90)90114-6

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Anti-topoisomerase I autoantibodies (anti-topo I, anti-Scl-70) are associated with proximal scleroderma and are of prognostic significance in patients with Raynaud's disease. To establish a highly sensitive and specific system for the detection of anti-topo I, we have investigated sera from 409 patients and controls by Ouchterlony gel diffusion, Western immunoblot on chromosome proteins, and solid-phase enzyme-linked immunosorbent assay (ELISA) with purified topoisomerase I as antigen. The ELISA was more sensitive than the gel diffusion technique and was more specific than the Western immunoblot, while the immunoblot may identify additional autoantibodies. © 1990.

引用

页码：399 / 410

页数：12

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