CHLOROPLAST DNA RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS (RFLPS) IN MUSA SPECIES

被引:49
作者
GAWEL, NJ [1 ]
JARRET, RL [1 ]
机构
[1] USDA ARS,REG PLANT INTRODUCT STN,GRIFFIN,GA 30223
关键词
BANANA; CPDNA; PLANTAIN; SYSTEMATICS;
D O I
10.1007/BF00224990
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Taxonomic and phylogenetic determinations within the genus Musa are established using a numerical, morphology-based scoring system. However, within this system, the classification and relationships of some types are disputed. The application of chloroplast DNA (cpDNA) restriction fragment length polymorphism (RFLP) analysis to Musa taxonomy provided valuable, supplemental information about the classification of, and relationships between, Musa species and subspecies. Whole-cell DNA was extracted from lyophilized Musa leaf-blade tissue and digested with various restriction enzymes, Southern blotted onto nylon membranes, and probed using radioactively labeled heterologous orchid cpDNA fragments. Phylogenies were inferred from cpDNA RFLP patterns using PAUP software. The relationships between most species examined were as expected; however, some species (M. beccarii and M. basjoo) did not conform to the conventional morphology-based phylogeny.
引用
收藏
页码:783 / 786
页数:4
相关论文
共 19 条
[1]  
ARGENT G C G, 1976, Notes from the Royal Botanic Garden Edinburgh, V35, P77
[2]  
BONNER J W, 1974, Hortscience, V9, P325
[3]  
GAWEL N, 1991, EUPHYTICA, V52, P19
[4]   ENZYME POLYMORPHISM IN MUSA-ACUMINATA COLLA [J].
JARRET, RL ;
LITZ, RE .
JOURNAL OF HEREDITY, 1986, 77 (03) :183-188
[5]   ISOZYMES AS GENETIC-MARKERS IN BANANAS AND PLANTAINS [J].
JARRET, RL ;
LITZ, RE .
EUPHYTICA, 1986, 35 (02) :539-549
[6]  
Maniatis T., 1982, MOL CLONING
[7]   A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].
MURASHIGE, T ;
SKOOG, F .
PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497
[8]   RAPID ISOLATION OF HIGH MOLECULAR-WEIGHT PLANT DNA [J].
MURRAY, MG ;
THOMPSON, WF .
NUCLEIC ACIDS RESEARCH, 1980, 8 (19) :4321-4325
[9]  
RIVERA FN, 1983, PARADISIACA, V6, P7
[10]  
ROWE PR, 1981, GENETIC ENG CROP IMP, P66