CO-AMPLIFICATION OF SPECIFIC SEQUENCES OF HCV AND HIV-1 GENOMES BY USING THE POLYMERASE CHAIN-REACTION ASSAY - A POTENTIAL TOOL FOR THE SIMULTANEOUS DETECTION OF HCV AND HIV-1

被引:13
作者
NEDJAR, S [1 ]
BISWAS, RM [1 ]
HEWLETT, IK [1 ]
机构
[1] US FDA,CTR BIOL EVALUAT & RES,DIV TRANSFUS SCI,RETROVIROL LAB,BETHESDA,MD 20014
关键词
HCV; HIV-1; PCR; CO-AMPLIFICATION; DIAGNOSIS;
D O I
10.1016/0166-0934(91)90071-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A rapid and simple method using the polymerase chain reaction (PCR) was devised for the co-amplification and simultaneous detection of hepatitis C virus (HCV) and human immunodeficiency virus type 1 (HIV-1) specific sequences in the same serum sample. Genomic RNA was extracted from 13 blood donor sera that were reactive in ELISA for both anti-HCV and anti-HIV-1. The extracted RNA was reverse transcribed into cDNA and amplified using nested primer pairs (SN01 and SN04; SN02 and SN03) based on the HCV prototype sequence of clones 37b and 81, and SK 38/39 for HIV-1 simultaneously. PCR products were analyzed by liquid hybridization or Southern blot hybridization with P-32 end-labeled oligonucleotide probes from the regions between the primer pairs, excluding the primer sequences. HCV-RNA was detected in all 13 (100%) samples tested; HIV-RNA was detected in 11 (85%) samples. The ability to co-amplify specific sequences from two different viral genomes in the same reaction mixture offers the possibility of simultaneous detection and diagnosis of more than one viral agent in serum samples of infected individuals.
引用
收藏
页码:297 / 304
页数:8
相关论文
共 14 条
[1]   ISOLATION OF A T-LYMPHOTROPIC RETROVIRUS FROM A PATIENT AT RISK FOR ACQUIRED IMMUNE-DEFICIENCY SYNDROME (AIDS) [J].
BARRESINOUSSI, F ;
CHERMANN, JC ;
REY, F ;
NUGEYRE, MT ;
CHAMARET, S ;
GRUEST, J ;
DAUGUET, C ;
AXLERBLIN, C ;
VEZINETBRUN, F ;
ROUZIOUX, C ;
ROZENBAUM, W ;
MONTAGNIER, L .
SCIENCE, 1983, 220 (4599) :868-871
[2]   HEPATITIS-C VIRUS TRANSMISSION BY MONOCLONAL-ANTIBODY PURIFIED FACTOR-VIII CONCENTRATE [J].
BERNTORP, E ;
NILSSON, IM ;
LJUNG, R ;
WIDELL, A .
LANCET, 1990, 335 (8704) :1531-1532
[3]   ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME [J].
CHOO, QL ;
KUO, G ;
WEINER, AJ ;
OVERBY, LR ;
BRADLEY, DW ;
HOUGHTON, M .
SCIENCE, 1989, 244 (4902) :359-362
[4]  
HEWLETT IK, 1988, J CLIN IMMUNOASSAY, V11, P161
[5]  
HEWLETT IK, 1989, ONCOGENE, V4, P1145
[6]   AN ASSAY FOR CIRCULATING ANTIBODIES TO A MAJOR ETIOLOGIC VIRUS OF HUMAN NON-A, NON-B-HEPATITIS [J].
KUO, G ;
CHOO, QL ;
ALTER, HJ ;
GITNICK, GL ;
REDEKER, AG ;
PURCELL, RH ;
MIYAMURA, T ;
DIENSTAG, JL ;
ALTER, MJ ;
STEVENS, CE ;
TEGTMEIER, GE ;
BONINO, F ;
COLOMBO, M ;
LEE, WS ;
KUO, C ;
BERGER, K ;
SHUSTER, JR ;
OVERBY, LR ;
BRADLEY, DW ;
HOUGHTON, M .
SCIENCE, 1989, 244 (4902) :362-364
[7]   IDENTIFICATION OF HUMAN-IMMUNODEFICIENCY-VIRUS SEQUENCES BY USING INVITRO ENZYMATIC AMPLIFICATION AND OLIGOMER CLEAVAGE DETECTION [J].
KWOK, S ;
MACK, DH ;
MULLIS, KB ;
POIESZ, B ;
EHRLICH, G ;
BLAIR, D ;
FRIEDMANKIEN, A ;
SNINSKY, JJ .
JOURNAL OF VIROLOGY, 1987, 61 (05) :1690-1694
[8]   HEPATITIS-C ANTIBODY AND CHRONIC LIVER-DISEASE IN HEMOPHILIA [J].
MAKRIS, M ;
PRESTON, FE ;
TRIGER, DR ;
UNDERWOOD, JCE ;
CHOO, QL ;
KUO, G ;
HOUGHTON, M .
LANCET, 1990, 335 (8698) :1117-1119
[9]  
MENDENHALL CL, 1986, NEW ENGL J MED, V314, P921, DOI 10.1056/NEJM198604033141412
[10]   DNA AMPLIFICATION FOR DIRECT DETECTION OF HIV-1 IN DNA OF PERIPHERAL-BLOOD MONONUCLEAR-CELLS [J].
OU, CY ;
KWOK, S ;
MITCHELL, SW ;
MACK, DH ;
SNINSKY, JJ ;
KREBS, JW ;
FEORINO, P ;
WARFIELD, D ;
SCHOCHETMAN, G .
SCIENCE, 1988, 239 (4837) :295-297