SELECTION OF VARIANTS OF THE TEM-1 BETA-LACTAMASE, ENCODED BY A PLASMID OF CLINICAL-ORIGIN, WITH INCREASED RESISTANCE TO BETA-LACTAMASE INHIBITORS

被引:17
作者
THOMSON, CJ
AMYES, SGB
机构
[1] Department of Medical Microbiology, Medical School, Univesity of Edinburgh, Edinburgh EH8 9AG, Teviot Place
基金
英国惠康基金;
关键词
D O I
10.1093/jac/31.5.655
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
A TEM-1 derived variant β lactamase with increased resistance to β lactamase inhibitors was selected in vitro. The variant β lactamase was obtained from the parent strain Escherichia coli J62-2 containing resistance plasmid R1 which encodes the TEM-1 β lactamase. The variants were obtained by repeated subculture of E. coli J62-2 (R1) for five days in the presence of sub-inhibitory concentrations of amoxycillin plus clavulanic acid. Comparison of this variant β lactamase with a clinically isolated TEM derived β lactamase, with increased resistance to β lactamase inhibitors, suggests that they are the same enzyme. Both β lactamases have the same pI at 5·25 and have similar ID50 values for the β lactamase inhibitors clavulanic acid, sulbactam and tazobactam. © 1993 The British Society for Antimicrobial Chemotherapy.
引用
收藏
页码:655 / 664
页数:10
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