A PHYTOCHROME PHOTOTRANSFORMATION STUDY USING 2-LASER 2-COLOR FLASH-PHOTOLYSIS - ANALYSIS OF THE DECAY MECHANISM OF I(700)

被引：35

作者：

SCURLOCK, RD ^{[1
]}

EVANS, CH ^{[1
]}

BRASLAVSKY, SE ^{[1
]}

SCHAFFNER, K ^{[1
]}

机构：

[1] MAX PLANCK INST STRAHLENCHEM,W-4330 MULHEIM,GERMANY

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1993年 / 58卷 / 01期

关键词：

D O I：

10.1111/j.1751-1097.1993.tb04910.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mechanism of I700 decay, representing a nearly event in the phytochrome P(r) --> P(fr) phototransformation, was reanalyzed in the microsecond range by conventional laser flash photolysis as well as by two-laser/two-color flash photolysis. Three kinetic models that might describe the I700 decay mechanism following P(r) excitation were considered: a parallel, a sequential, and an equilibrium model. These models were used to mathematically simulate both the one- and two-laser flash experiments in an effort to select the model best describing the I700 decay. The sequential model could be excluded already on the basis of the one-laser flash photolysis results alone. Discussion of the two-laser/two-color flash results in the context of the equilibrium and the parallel models is presented.

引用

页码：106 / 115

页数：10

共 25 条

[1] LASER FLASH-PHOTOLYSIS OF 124-KILODALTON OAT PHYTOCHROME IN H2O AND D2O SOLUTIONS - FORMATION AND DECAY OF THE I700 INTERMEDIATES [J].