ATRIAL-NATRIURETIC-FACTOR ACTIVATES MEMBRANE-BOUND GUANYLATE-CYCLASE OF CHIEF CELLS

被引：7

作者：

CHERNER, JA ^{[1
]}

SINGH, G ^{[1
]}

NAIK, L ^{[1
]}

机构：

[1] VANDERBILT UNIV,MED CTR,SCH MED,NASHVILLE,TN 37232

来源：

LIFE SCIENCES | 1990年 / 47卷 / 07期

关键词：

D O I：

10.1016/0024-3205(90)90579-G

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The present study examined the effect of atrial natriuretic factor (ANF) on cGMP generation by dispersed chief cells from guinea pig stomach. ANF caused a rapid dose-dependent increase in cGMP, a 7-fold increase in cGMP caused by 1 μM ANF, with or without 3-isobutyl-1-methylxanthine present. Methylene blue reduced cGMP in response to nitroprusside but not ANF. Guanylate cyclase activity of a chief cell membrane fraction doubled in response to ANF, but was not affected by nitroprusside. ANF had no effect on guanylate cyclase activity of the soluble fraction of lysed chief cells. Dose-response curves for whole cell cGMP production and membrane guanylate cyclase activity in response to ANF were closely related. These data indicate that ANF increases chief cell cGMP production by activating particulate guanylate cyclase, providing functional evidence that chief cells possess surface membrane receptors for ANF. © 1990.

引用

页码：669 / 677

页数：9

共 17 条

[1] ATRIAL NATRIURETIC FACTOR BINDING-SITES IN THE JEJUNUM [J].