ANTIBODY-CATALYZED REARRANGEMENT OF THE PEPTIDE-BOND

被引：34

作者：

GIBBS, RA

TAYLOR, S

BENKOVIC, SJ

机构：

[1] Department of Chemistry, Pennsylvania State University, 152 Davey Laboratory, University Park

来源：

SCIENCE | 1992年 / 258卷 / 5083期

关键词：

D O I：

10.1126/science.1439788

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The generation of antibodies from a bifunctional cyclic phosphinate transition-state analog provided agents capable of efficiently catalyzing both steps of the overall conversion of a substrate containing an asparaginyl-glycyl sequence through a succinimide intermediate to the products aspartyl-glycyl and the rearranged isoaspartyl-glycyl sequence. This reaction provides a potential means in addition to amide cleavage for the deactivation of protein or peptide biological functions in vivo.

引用

收藏

页码：803 / 805

页数：3

相关论文

共 17 条

[1] THE MECHANISM OF IRREVERSIBLE ENZYME INACTIVATION AT 100-DEGREES-C [J].

AHERN, TJ ;

KLIBANOV, AM .

SCIENCE, 1985, 228 (4705) :1280-1284

[2] PROPENSITY FOR SPONTANEOUS SUCCINIMIDE FORMATION FROM ASPARTYL AND ASPARAGINYL RESIDUES IN CELLULAR PROTEINS [J].

CLARKE, S .

INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, 1987, 30 (06) :808-821

[3]

GIBBS RB, UNPUB

[4] CATALYTIC ANTIBODIES WITH LIPASE ACTIVITY AND R-SUBSTRATE OR S-SUBSTRATE SELECTIVITY [J].

JANDA, KD ;

BENKOVIC, SJ ;

LERNER, RA .

SCIENCE, 1989, 244 (4903) :437-440

[5] TERTIARY STRUCTURE IS A PRINCIPAL DETERMINANT TO PROTEIN DEAMIDATION [J].

KOSSIAKOFF, AA .

SCIENCE, 1988, 240 (4849) :191-194

[6] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[7] AT THE CROSSROADS OF CHEMISTRY AND IMMUNOLOGY - CATALYTIC ANTIBODIES [J].

LERNER, RA ;

BENKOVIC, SJ ;

SCHULTZ, PG .

SCIENCE, 1991, 252 (5006) :659-667

[8]

MODRITZER K, 1975, SYN REACT INORG MET, V5, P45

[9] A STEREOSPECIFIC CYCLIZATION CATALYZED BY AN ANTIBODY [J].

NAPPER, AD ;

BENKOVIC, SJ ;

TRAMONTANO, A ;

LERNER, RA .

SCIENCE, 1987, 237 (4818) :1041-1043

[10] MOLECULAR DISSECTION OF RAUSCHER VIRUS GP70 BY USING MONOCLONAL-ANTIBODIES - LOCALIZATION OF ACQUIRED SEQUENCES OF RELATED ENVELOPE GENE RECOMBINANTS [J].

NIMAN, HL ;

ELDER, JH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (08) :4524-4528