RIBOSOME-BINDING SITES ON CHLOROPLAST RBCL AND PSBA MESSENGER-RNAS AND LIGHT-INDUCED INITIATION OF D1 TRANSLATION

被引:55
作者
KIM, J [1 ]
MULLET, JE [1 ]
机构
[1] TEXAS A&M UNIV, DEPT BIOCHEM & BIOPHYS, COLLEGE STN, TX 77843 USA
关键词
CHLOROPLAST; LIGHT INDUCTION; PSBA; RBCL; TRANSLATION INITIATION;
D O I
10.1007/BF00043872
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chloroplast ribosome-binding sites were identified on the plastid rbcL and psbA mRNAs using toeprint analysis. The rbcL translation initiation domain is highly conserved and contains a prokaryotic Shine-Dalgarno (SD) sequence (GGAGG) located 4 to 12 nucleotides upstream of the initiator AUG. Toeprint analysis of rbcL mRNA associated with plastid polysomes revealed strong toeprint signals 15 nucleotides downstream from the AUG indicating ribosome binding at the translation initiation site. Escherichia coli 30S ribosomes generated similar toeprint signals when mixed with rbcL mRNA in the presence of initiator tRNA. These results indicate that plastid SD sequences are functional in chloroplast translation initiation. The psbA initiator region lacks a SD sequence within 12 nucleotides of the initiator AUG. However, toeprint analysis of soluble and membrane polysome-associated psbA mRNA revealed ribosomes bound to the initiator region. E. coli 30S ribosomes did not associate with the psbA translation initiation region. E. coli and chloroplast ribosomes bind to an upstream region which contains a conserved SD-like sequence. Therefore, translation initiation on psbA mRNA may involve the transient binding of chloroplast ribosomes to this upstream SD-like sequence followed by scanning to localize the initiator AUG. Illumination 8-day-old dark-grown barley seedlings caused an increase in polysome-associated psbA mRNA and the abundance of initiation complexes bound to psbA mRNA. These results demonstrate that light modulates D1 translation initiation in plastids of older dark-grown barley seedlings.
引用
收藏
页码:437 / 448
页数:12
相关论文
共 37 条
[1]  
ADIR N, 1990, J BIOL CHEM, V265, P12563
[2]   RIBOSOME-THYLAKOID ASSOCIATION IN PEAS - INFLUENCE OF ANOXIA [J].
ALSCHERHERMAN, R ;
JAGENDORF, AT ;
GRUMET, R .
PLANT PHYSIOLOGY, 1979, 64 (02) :232-235
[3]   PLASTID GENES ENCODING THE TRANSCRIPTION TRANSLATION APPARATUS ARE DIFFERENTIALLY TRANSCRIBED EARLY IN BARLEY (HORDEUM-VULGARE) CHLOROPLAST DEVELOPMENT - EVIDENCE FOR SELECTIVE STABILIZATION OF PSBA MESSENGER-RNA [J].
BAUMGARTNER, BJ ;
RAPP, JC ;
MULLET, JE .
PLANT PHYSIOLOGY, 1993, 101 (03) :781-791
[4]   MESSENGER-RNAS ENCODING RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE REMAIN BOUND TO POLYSOMES BUT ARE NOT TRANSLATED IN AMARANTH SEEDLINGS TRANSFERRED TO DARKNESS [J].
BERRY, JO ;
CARR, JP ;
KLESSIG, DF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (12) :4190-4194
[5]   TRANSCRIPTIONAL AND POST-TRANSCRIPTIONAL REGULATION OF RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE GENE-EXPRESSION IN LIGHT-GROWN AND DARK-GROWN AMARANTH COTYLEDONS [J].
BERRY, JO ;
NIKOLAU, BJ ;
CARR, JP ;
KLESSIG, DF .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (09) :2238-2246
[6]   TRANSLATIONAL REGULATION OF LIGHT-INDUCED RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE GENE-EXPRESSION IN AMARANTH [J].
BERRY, JO ;
NIKOLAU, BJ ;
CARR, JP ;
KLESSIG, DF .
MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (07) :2347-2353
[7]   LIGHT-MEDIATED CONTROL OF TRANSLATIONAL INITIATION OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE IN AMARANTH COTYLEDONS [J].
BERRY, JO ;
BREIDING, DE ;
KLESSIG, DF .
PLANT CELL, 1990, 2 (08) :795-803
[8]   THE COMPONENTS OF THE PLASTID RIBOSOME ARE NOT ACCUMULATED SYNCHRONOUSLY DURING THE EARLY DEVELOPMENT OF SPINACH PLANTS [J].
BISANZSEYER, C ;
LI, YF ;
SEYER, P ;
MACHE, R .
PLANT MOLECULAR BIOLOGY, 1989, 12 (02) :201-211
[9]   TRANSLATION OF CHLOROPLAST-ENCODED MESSENGER-RNA - POTENTIAL INITIATION AND TERMINATION SIGNALS [J].
BONHAMSMITH, PC ;
BOURQUE, DP .
NUCLEIC ACIDS RESEARCH, 1989, 17 (05) :2057-2080
[10]   SEQUENCE AND TRANSCRIPT MAP OF BARLEY CHLOROPLAST PSBA GENE [J].
BOYER, SK ;
MULLET, JE .
NUCLEIC ACIDS RESEARCH, 1988, 16 (16) :8184-8184