DNA-DAMAGE IN HUMAN RESPIRATORY-TRACT EPITHELIAL-CELLS - DAMAGE BY GAS-PHASE CIGARETTE-SMOKE APPARENTLY INVOLVES ATTACK BY REACTIVE NITROGEN SPECIES IN ADDITION TO OXYGEN RADICALS

Treatment of human respiratory tract tracheobronchial epithelial cells with gas-phase cigarette smoke led to dose-dependent DNA strand breakage that was highly correlated with multiple chemical modifications of all four DNA bases. The pattern of base damage suggests attack by hydroxyl radicals (OH.). However, by far the most important base damage in quantitative terms was formation of xanthine and hypoxanthine, presumably resulting from deamination of guanine and adenine respectively. Hence, DNA damage by cigarette smoke may invoke reactive nitrogen species as well as reactive oxygen species.