CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THERMOTOGA-NEAPOLITANA GENES-CODING FOR ENZYMES OF CARBOHYDRATE SUBSTRATE DEGRADATION

被引：38

作者：

DAKHOVA, ON

KUREPINA, NE

ZVERLOV, VV

SVETLICHNYI, VA

VELIKODVORSKAYA, GA

机构：

[1] Institute of Molecular Genetics, Russian Academy of Science, Moscow, 123182

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 194卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1974

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A genomic library of thermophilic anaerobic eubacterium Thermatoga neapolitana was constructed in E. coli using the pTZ19R plasmid vector. Some groups of recombinant clones with different cellulase activities were revealed: clones carrying genes for an 1,4-beta-glucanases, 1,3-beta-glucanases, beta-xylanases, beta-glucosidases and beta-xylosidases. One clone possessing avicelase activity was obtained. Some clones were selected with amylolytic activities toward amylose, amylopectin and pullulan. © 1993 Academic Press, Inc.

引用

页码：1359 / 1364

页数：6

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