ISOLATION AND FRACTIONATION OF RETROVIRAL TRANSFER-RNAS

被引：8

作者：

JIANG, M ^{[1
]}

PARNIAK, MA ^{[1
]}

KLEIMAN, L ^{[1
]}

机构：

[1] SIR MORTIMER B DAVIS JEWISH HOSP,LADY DAVIS INST MED RES,3755 COTE STE CATHERINE RD,MONTREAL H3T 1E2,QUEBEC,CANADA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1992年 / 38卷 / 02期

关键词：

MOUSE MAMMARY TUMOR VIRUS; TRANSFER RNA FRACTIONATION; COLUMN CHROMATOGRAPHY;

D O I：

10.1016/0166-0934(92)90111-P

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies concerning the analysis of retroviral tRNA populations involved intracellular metabolic labeling of RNA, followed by the isolation of viral RNA and lengthy sucrose gradient centrifugation for the separation of tRNAs found in various viral compartments. A more rapid, convenient, and safer method for achieving similar aims is described. Isolated total viral RNA is end-labeled in vitro, and tRNA subgroups are fractionated using commercial Nucleobond AX-20 mini columns. 2-D PAGE analysis of mouse mammary tumor virus tRNA fractionated in this way yields gel patterns similar to those obtained with previously described methods.

引用

页码：205 / 213

页数：9

共 16 条

[1] REACTIONS AT TERMINI OF TRANSFER-RNA WITH T4 RNA LIGASE [J].