PROPERTIES OF ASPERGILLUS-JAPONICUS BETA-FRUCTOFURANOSIDASE IMMOBILIZED ON POROUS SILICA

被引：15

作者：

HAYASHI, S

MATSUZAKI, K

INOMATA, Y

TAKASAKI, Y

IMADA, K

机构：

[1] Department of Industrial Chemistry, Faculty of Engineering, Miyazaki University, Miyazaki, 889-21

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1993年 / 9卷 / 02期

关键词：

ASPERGILLUS; BETA-FRUCTOFURANOSIDASE; FRUCTO-OLIGOSACCHARIDE; IMMOBILIZATION; POROUS SILICA;

D O I：

10.1007/BF00327841

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Beta-Fructofuranosidase from Aspergillus japonicus MU-2, which produces fructo-oligosaccharides (1-kestose: O-beta-D-fructofuranosyl-(2 --> 1)-beta-D-fructofuranosyl alpha-D-glucopyranoside); and nystose: O-beta-D-fructofuranosyl-(2 --> 1)-beta-D-fructofuranosyl-(2 --> 1)-beta-D-fructofuranosyl alpha-D-glucopyranoside) from sucrose, was immobilized, covalently with glutaraldehyde onto alkylamine porous silica, at high efficiency (64%). Optimum pore diameter of porous silica for immobilization of the enzyme was 91.7 run. After immobilization, the enzyme's stabilities to temperature, metal ions and proteolysis were improved, while its optimum pH and temperature were unchanged. The highest efficiency of continuous production of fructo-oligosaccharides (more than 60%), using a column packed with the immobilized enzyme, was obtained at 40% to 50% (w/v) sucrose. The half-life of the column during long-term continuous operation at 55-degrees-C was 29 days.

引用

页码：216 / 220

页数：5

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