RANDOM INSERTION OF TN4560 IN STREPTOMYCES-LIVIDANS AND STREPTOMYCES-AVERMITILIS

被引：3

作者：

IRNICH, S ^{[1
]}

CULLUM, J ^{[1
]}

机构：

[1] UNIV KAISERSLAUTERN,LB GENET,POSTFACH 3049,D-67653 KAISERSLAUTERN,GERMANY

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 09期

关键词：

D O I：

10.1007/BF00131753

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pulsed-field gel electrophoresis (PFGE) was used to show that insertions of transposon Tn4560 in Streptomyces lividans 66 and the avermectin-producer S. avermitilis are randomly distributed. DNA from the latter strain suffered. degradation during electrophoresis that could be avoided by modification of the buffer. Inverse PCR primers were developed for Tn4560.

引用

页码：895 / 900

页数：6

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