TRANSFORMATION OF THE YEAST SACCHAROMYCES-KLUYVERI BY SACCHAROMYCES-CEREVISIAE-BASED PLASMIDS

被引：11

作者：

FUJIMURA, HA

机构：

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 82卷 / 02期

关键词：

TRANSFORMATION; SACCHAROMYCES-KLUYVERI; SACCHAROMYCES-CEREVISIAE; PLASMID; URACIL AUXOTROPH;

D O I：

10.1111/j.1574-6968.1991.tb04856.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

For the transformation of the yeast Saccharomyces kluyveri, ura3 mutants were obtained by 5-fluoro-orotic acid selection. By utilizing the method based on treatment of intact cells with alkali cations, the ura3 strains of S. kluyveri were transformed by Saccharomyces cerevisiae-based plasmids. In the transformed cells, a S. cerevisiae centromere-based plasmid was stably replicated autonomously. Thus, this system will permit the study of gene expression and its regulation in S. kluyveri in relationship to that in S. cerevisiae.

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页码：149 / 152

页数：4

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