ENZYMATIC-SYNTHESIS AND STRUCTURE OF PRECORRIN-3, A TRIMETHYLDIPYRROCORPHIN INTERMEDIATE IN VITAMIN-B12 BIOSYNTHESIS

被引：48

作者：

WARREN, MJ ^{[1
]}

ROESSNER, CA ^{[1
]}

OZAKI, SI ^{[1
]}

STOLOWICH, NJ ^{[1
]}

SANTANDER, PJ ^{[1
]}

SCOTT, AI ^{[1
]}

机构：

[1] TEXAS A&M UNIV SYST,DEPT CHEM,CTR BIOL NMR,COLLEGE STN,TX 77843

来源：

BIOCHEMISTRY | 1992年 / 31卷 / 02期

关键词：

D O I：

10.1021/bi00117a043

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The trimethylated intermediate of vitamin B-12 (corrin) biosynthesis, precorrin-3, was produced from various C-13-enriched isotopomers of 5-aminolevulinic acid (ALA), using a multiple-enzyme system containing ALA dehydratase, porphobilinogen deaminase, uro'gen III synthetase, and the S-adenosyl-L-methionine-(SAM)-dependent uro'gen III methyltransferase (M-1) and precorrin-2 methyltransferase (M-2) in the presence of [C-13]SAM. Structural analysis of the resulting product, precorrin-3, reveals a close similarity to precorrin-2 but with several subtle differences in the conjugated array of C = C and C = N bonds which reflect the presence of the new C-methyl group at C20 and its influence on the electronic distribution in the dipyrrocorphin chromophore. The implications of this structure for corrin biosynthesis are discussed.

引用

页码：603 / 609

页数：7

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