CAMBIAL TISSUE-CULTURE AND SUBSEQUENT SHOOT REGENERATION FROM MATURE BLACK LOCUST (ROBINIA-PSEUDOACACIA L)

被引：3

作者：

HAN, KH ^{[1
]}

KEATHLEY, DE ^{[1
]}

GORDON, MP ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV,DEPT FORESTRY,E LANSING,MI 48824

来源：

PLANT CELL REPORTS | 1993年 / 12卷 / 04期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Callus initiation and subsequent shoot regeneration were obtained from cambial tissue of mature black locust trees. Shoot regeneration was highly tree specific, and was only achieved when calli derived from a particular genotype (tree #1) were transferred to medium containing 6-benzylaminopurine. The studies described here show that the immediate use of a mature genotype as starting material for tissue cultural manipulation is possible by using cambial explants with the proper in vitro conditions. We also found that stems could be stored at 4-degrees-C for 8 months prior to culture initiation, with no decline in die viability of the cultures. Tissue sources can thus be continuously available.

引用

页码：185 / 188

页数：4

共 13 条

[1]

[Anonymous], 1991, PRINCIPLES PROCEDURE

[2] MASS CLONAL PROPAGATION INVITRO OF ROBINIA-PSEUDOACACIA L (BLACK LOCUST)CV JASZKISERI [J].