COOPERATIVE REGULATION OF ENDOCYTOSIS BY 3 RAB5 ISOFORMS

被引：142

作者：

BUCCI, C

LUTCKE, A

STEELEMORTIMER, O

OLKKONEN, VM

DUPREE, P

CHIARIELLO, M

BRUNI, CB

SIMONS, K

ZERIAL, M

机构：

[1] CNR,DIPARTIMENTO BIOL & PATOL CELLULARE & MOLEC L CAL,I-80131 NAPLES,ITALY

[2] CNR,CTR ENDOCRINOL & ONCOL SPERIMENTALE,I-80131 NAPLES,ITALY

[3] EUROPEAN MOLEC BIOL LAB,D-69012 HEIDELBERG,GERMANY

[4] NATL PUBL HLTH INST,DEPT BIOCHEM,SF-00300 HELSINKI,FINLAND

[5] UNIV CAMBRIDGE,DEPT PLANT SCI,CAMBRIDGE CB2 3EA,ENGLAND

来源：

FEBS LETTERS | 1995年 / 366卷 / 01期

关键词：

RAB PROTEIN; RAB5; ISOFORM; ENDOCYTOSIS; EUKARYOTIC CELL;

D O I：

10.1016/0014-5793(95)00477-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rab proteins are small GTPases involved in the regulation of membrane traffic, Rab5a has been shown to regulate transport in the early endocytic pathway. Here we report the isolation of cDNA clones encoding two highly related isoforms, Rab5b and Rab5c, The two proteins share with Rab5a all the structural features required for regulation of endocytosis. Rab5b and Rab5c colocalize with the both transferrin receptor and Rab5a, stimulate the homotypic fusion between early endosomes in vitro and increase the rate of endocytosis when overexpressed in vivo, These data demonstrate that three Rab5 isoforms cooperate in the regulation of endocytosis in eukaryotic cells.

引用

页码：65 / 71

页数：7

共 42 条

[1] CLONING OF A RAB3 ISOTYPE PREDOMINATELY EXPRESSED IN ADIPOCYTES
BALDINI, G
HOHL, T
LIN, HY
LODISH, HF
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) : 5049 - 5052
[2] INTERACTIONS OF 3 DOMAINS DISTINGUISHING THE RAS-RELATED GTP-BINDING PROTEINS YPT1 AND SEC4
BRENNWALD, P
NOVICK, P
[J]. NATURE, 1993, 362 (6420) : 560 - 563
[3] THE SMALL GTPASE RAB5 FUNCTIONS AS A REGULATORY FACTOR IN THE EARLY ENDOCYTIC PATHWAY
BUCCI, C
PARTON, RG
MATHER, IH
STUNNENBERG, H
SIMONS, K
HOFLACK, B
ZERIAL, M
[J]. CELL, 1992, 70 (05) : 715 - 728
[4] RAB5A IS A COMMON COMPONENT OF THE APICAL AND BASOLATERAL ENDOCYTIC MACHINERY IN POLARIZED EPITHELIAL-CELLS
BUCCI, C
WANDINGERNESS, A
LUTCKE, A
CHIARIELLO, M
BRUNI, CB
ZERIAL, M
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (11) : 5061 - 5065
[5] LOCALIZATION OF LOW-MOLECULAR-WEIGHT GTP BINDING-PROTEINS TO EXOCYTIC AND ENDOCYTIC COMPARTMENTS
CHAVRIER, P
PARTON, RG
HAURI, HP
SIMONS, K
ZERIAL, M
[J]. CELL, 1990, 62 (02) : 317 - 329
[6] MOLECULAR-CLONING OF YPT1/SEC4-RELATED CDNAS FROM AN EPITHELIAL-CELL LINE
CHAVRIER, P
VINGRON, M
SANDER, C
SIMONS, K
ZERIAL, M
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (12) : 6578 - 6585
[7] THE COMPLEXITY OF THE RAB AND RHO GTP-BINDING PROTEIN SUBFAMILIES REVEALED BY A PCR CLONING APPROACH
CHAVRIER, P
SIMONS, K
ZERIAL, M
[J]. GENE, 1992, 112 (02) : 261 - 264
[8] HYPERVARIABLE C-TERMINAL DOMAIN OF RAB PROTEINS ACTS AS A TARGETING SIGNAL
CHAVRIER, P
GORVEL, JP
STELZER, E
SIMONS, K
GRUENBERG, J
ZERIAL, M
[J]. NATURE, 1991, 353 (6346) : 769 - 772
[9] ASSOCIATION OF THE GTP-BINDING PROTEIN-RAB3A WITH BOVINE ADRENAL CHROMAFFIN GRANULES
DARCHEN, F
ZAHRAOUI, A
HAMMEL, F
MONTEILS, MP
TAVITIAN, A
SCHERMAN, D
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (15) : 5692 - 5696
[10] THE INVOLVEMENT OF THE SMALL GTP-BINDING PROTEIN RAB5A IN NEURONAL ENDOCYTOSIS
DEHOOP, MJ
HUBER, LA
STENMARK, H
WILLIAMSON, E
ZERIAL, M
PARTON, RG
DOTTI, CG
[J]. NEURON, 1994, 13 (01) : 11 - 22

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