MEASUREMENT OF CROSS RELAXATION BETWEEN 2 SELECTED NUCLEI BY SYNCHRONOUS NUTATION OF MAGNETIZATION IN NUCLEAR-MAGNETIC-RESONANCE

被引:29
作者
BURGHARDT, I [1 ]
KONRAT, R [1 ]
BOULAT, B [1 ]
VINCENT, SJF [1 ]
BODENHAUSEN, G [1 ]
机构
[1] UNIV LAUSANNE,CHIM PHYS SECT,CH-1005 LAUSANNE,SWITZERLAND
关键词
D O I
10.1063/1.464287
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
A novel technique is described that allows one to measure cross-relaxation rates (Overhauser effects) between two selected nuclei in high-resolution NMR. The two chosen sites are irradiated simultaneously with the sidebands of an amplitude-modulated radio-frequency field, so that their magnetization vectors are forced to undergo a simultaneous motion, which is referred to as ''synchronous nutation.'' From the time-dependence observed for different initial conditions, one may derive cross-relaxation rates, and hence determine internuclear distances. The scalar interactions between the selected spins and other spins belonging to the same coupling network are effectively decoupled. Furthermore, cross relaxation to other spins in the environment does not affect the transient response of the selected spins, which are therefore in effect isolated from their environment in terms of dipolar interactions. The method is particularly suitable to study cases where normal Overhauser effects are perturbed by spin-diffusion effects due to the presence of further spins. The technique is applied to the protein bovine pancreatic trypsin inhibitor.
引用
收藏
页码:1721 / 1736
页数:16
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