CLONING AND CHARACTERIZATION OF POLYKETIDE SYNTHASE GENES FOR JADOMYCIN-B BIOSYNTHESIS IN STREPTOMYCES-VENEZUELAE ISP5230

被引:82
作者
HAN, L [1 ]
YANG, KQ [1 ]
RAMALINGAM, E [1 ]
MOSHER, RH [1 ]
VINING, LC [1 ]
机构
[1] DALHOUSIE UNIV, DEPT BIOL, HALIFAX, NS B3H 4J1, CANADA
来源
MICROBIOLOGY-SGM | 1994年 / 140卷
关键词
STREPTOMYCES VENEZUELAE; JADOMYCINS; POLYKETIDE SYNTHASE GENE CLUSTER; GENE DISRUPTION;
D O I
10.1099/13500872-140-12-3379
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Hybridizing fragments in the genomic DNA of Streptomyces venezuelae ISP5230, which produces the jadomycin group of angucycline antibiotics, were detected by probing with actl DNA from Streptomyces coelicolor A3(2). The hybridizing regions were isolated from a 16.5 kb insert of S. venezuelae DNA recovered from a genomic library cloned in a ii replacement vector. Subcloning and sequencing of a 4.8 kb segment of the insert, containing regions hybridizing to actIII as well as actl, identified five open reading frames (ORFs). The deduced polypeptide products of the ORFs closely resemble in sequence the components of streptomycete type-ll polyketide synthases (PKSs): the ORF1 product corresponds to the ketoacyl synthase, and the ORF2 product to a polypeptide closely related to the ketoacyl synthase and involved in determining chain length; the ORF3 product matches the acyl carrier protein; ORF4 encodes a bifunctional cyclase/dehydrase; and ORF5 encodes a ketoreductase. Integration into the chromosomal DNA of a plasmid containing a segment of the ORF2-ORF4 region severely depressed jadomycin B biosynthesis; since the integrant showed no change in growth or spore pigmentation, the cloned PKS genes are presumed to encode enzymes in the pathway for jadomycin biosynthesis.
引用
收藏
页码:3379 / 3389
页数:11
相关论文
共 42 条
  • [1] EVIDENCE FOR A CHROMOSOMAL LOCATION OF THE GENES-CODING FOR CHLORAMPHENICOL PRODUCTION IN STREPTOMYCES-VENEZUELAE
    AHMED, ZU
    VINING, LC
    [J]. JOURNAL OF BACTERIOLOGY, 1983, 154 (01) : 239 - 244
  • [2] PLASMID TRANSFORMATION OF STREPTOMYCES-VENEZUELAE - MODIFIED PROCEDURES USED TO INTRODUCE THE GENE(S) FOR PARA-AMINOBENZOATE SYNTHASE
    AIDOO, DA
    BARRETT, K
    VINING, LC
    [J]. JOURNAL OF GENERAL MICROBIOLOGY, 1990, 136 : 657 - 662
  • [3] CHARACTERIZATION OF ACTI-HOMOLOGOUS DNA ENCODING POLYKETIDE SYNTHASE GENES FROM THE MONENSIN PRODUCER STREPTOMYCES-CINNAMONENSIS
    ARROWSMITH, TJ
    MALPARTIDA, F
    SHERMAN, DH
    BIRCH, A
    HOPWOOD, DA
    ROBINSON, JA
    [J]. MOLECULAR & GENERAL GENETICS, 1992, 234 (02): : 254 - 264
  • [4] JADOMYCIN, A NOVEL 8H-BENZ[B]OXAZOLO[3,2-F]PHENANTHRIDINE ANTIBIOTIC FROM STREPTOMYCES-VENEZUELAE ISP5230
    AYER, SW
    MCINNES, AG
    THIBAULT, P
    WALTER, JA
    DOULL, JL
    PARNELL, T
    VINING, LC
    [J]. TETRAHEDRON LETTERS, 1991, 32 (44) : 6301 - 6304
  • [5] BIOSYNTHESIS OF ANTHRAQUINONES BY INTERSPECIES CLONING OF ACTINORHODIN BIOSYNTHESIS GENES IN STREPTOMYCETES - CLARIFICATION OF ACTINORHODIN GENE FUNCTIONS
    BARTEL, PL
    ZHU, CB
    LAMPEL, JS
    DOSCH, DC
    CONNORS, NC
    STROHL, WR
    BEALE, JM
    FLOSS, HG
    [J]. JOURNAL OF BACTERIOLOGY, 1990, 172 (09) : 4816 - 4826
  • [6] ANALYSIS OF THE NUCLEOTIDE-SEQUENCE OF THE STREPTOMYCES-GLAUCESCENS TCML GENES PROVIDES KEY INFORMATION ABOUT THE ENZYMOLOGY OF POLYKETIDE ANTIBIOTIC BIOSYNTHESIS
    BIBB, MJ
    BIRO, S
    MOTAMEDI, H
    COLLINS, JF
    HUTCHINSON, CR
    [J]. EMBO JOURNAL, 1989, 8 (09) : 2727 - 2736
  • [7] THE RELATIONSHIP BETWEEN BASE COMPOSITION AND CODON USAGE IN BACTERIAL GENES AND ITS USE FOR THE SIMPLE AND RELIABLE IDENTIFICATION OF PROTEIN-CODING SEQUENCES
    BIBB, MJ
    FINDLAY, PR
    JOHNSON, MW
    [J]. GENE, 1984, 30 (1-3) : 157 - 166
  • [8] CLONING, SEQUENCING AND DEDUCED FUNCTIONS OF A CLUSTER OF STREPTOMYCES GENES PROBABLY ENCODING BIOSYNTHESIS OF THE POLYKETIDE ANTIBIOTIC FRENOLICIN
    BIBB, MJ
    SHERMAN, DH
    OMURA, S
    HOPWOOD, DA
    [J]. GENE, 1994, 142 (01) : 31 - 39
  • [9] CLONING AND DISRUPTION OF A FRAGMENT OF STREPTOMYCES-HALSTEDII DNA INVOLVED IN THE BIOSYNTHESIS OF A SPORE PIGMENT
    BLANCO, G
    PEREDA, A
    MENDEZ, C
    SALAS, JA
    [J]. GENE, 1992, 112 (01) : 59 - 65
  • [10] HYBRIDIZATION AND DNA-SEQUENCE ANALYSES SUGGEST AN EARLY EVOLUTIONARY DIVERGENCE OF RELATED BIOSYNTHETIC GENE SETS ENCODING POLYKETIDE ANTIBIOTICS AND SPORE PIGMENTS IN STREPTOMYCES SPP
    BLANCO, G
    BRIAN, P
    PEREDA, A
    MENDEZ, C
    SALAS, JA
    CHATER, KF
    [J]. GENE, 1993, 130 (01) : 107 - 116